Download Artificially Controllable Nanodevices Constructed by DNA by Yangyang Yang PDF

By Yangyang Yang

In this publication, the writer bargains mostly with subject matters: (1) single-molecule visualization of switching behaviors within the DNA nanoframe method using other forms of molecular switches by utilizing high-speed atomic strength microscope (AFM); (2) development of photocontrollable DNA nanostructures in programmed styles and direct visualization of the dynamic assembling approach. right here, high-speed AFM was once hired to watch the dynamic activities of unmarried molecules. in comparison to a standard single-molecule research technique, equivalent to fluorescence spectroscopy or electron microscopy, high-speed AFM makes attainable the real-time remark of molecule behaviors. DNA nanostructures have been designed and assembled as scaffolds to include biomolecules. The observations have been performed lower than strong stipulations with out complex pretreatment. furthermore, the photoresponsive molecules have been effectively assembled into round a hundred nm-sized DNA nanostructures. The assembly/disassembly of nanostructures might be regulated reversibly by way of photoirradiation. This publication explains how DNA origami has steadily develop into a great tool for the research of biochemical interactions in outlined nanospace. It additionally indicates the opportunity of DNA nanostructures performing as nanodevices for program in organic structures, serving as a great creation to simple DNA nanotechnology.

Show description

Read Online or Download Artificially Controllable Nanodevices Constructed by DNA Origami Technology: Photofunctionalization and Single-Molecule Analysis PDF

Similar nanostructures books

History of Strength of Materials

This wonderful old survey of the power of fabrics gains many references to the theories of elasticity and constitution. 245 figures.

Nano-society : pushing the boundaries of technology

Significant know-how shifts don't occur in a single day and infrequently are they the results of a unmarried leap forward discovery. Nowhere is that this truer than for the huge set of allowing applied sciences that we've got come to easily name "nanotechnology". instead of status at the shoulders of some highbrow giants, nanotechnologies are created through tens of hundreds of thousands of researchers and scientists engaged on minute and occasionally arcane facets in their fields of craftsmanship in components as varied as drugs, telecommunications, sun cells, filtration, coatings, or ever smaller transistors for digital units.

Metal nanoparticles for catalysis : advances and applications

Catalysis is a imperative subject in chemical transformation and effort conversion. due to the incredible achievements of colloidal chemistry and the synthesis of nanomaterials during the last 20 years, there have additionally been major advances in nanoparticle catalysis. Catalysis on diverse steel nanostructures with well-defined constructions and composition has been commonly studied.

Polymer Nanoclay Composites

There's a significant loss of primary wisdom and knowing at the interplay among a filler and the polymer matrix. by way of nanoscale fillers, comparable to layered silicates, carbon nanotubes, graphene or cellulose nanofibers it's much more very important to grasp actual structure-property relationships in addition to making a choice on the parameters influencing fabric habit.

Additional resources for Artificially Controllable Nanodevices Constructed by DNA Origami Technology: Photofunctionalization and Single-Molecule Analysis

Example text

5 High-Speed AFM Imaging of the dsDNAs in the DNA Frame High-speed AFM images were obtained on a fast-scanning AFM (Nano Live Vision, RIBM, Tsukuba, Japan) using a silicon nitride cantilever (Olympus BL-AC10EGS) cooperated with light source (Olympus U-RFL-T). The sample (2 μL) was absorbed on a freshly cleaved mica plate for 5 min at room temperature, and then washed with the buffer solution for the observation of single photo-switching directly. Scanning was performed in the same buffer solution using a tapping mode.

Three different dsDNAs carrying predetermined synthetic oligonucleotides modified by disulfide bonds (the method for the synthesizing can be seen in Chap. ) were all placed in parallel arrangements. 6), 1 mM EDTA, and 10 mM MgCl2 as following the previous study. 5 °C/min. 1 °C/min using a thermal cycler. The sample was purified using gel-filtration. The assembled structures were confirmed by AFM (Fig. 3b, c) after the removal of excessive dsDNAs and the yield of incorporation was evaluated (Fig. 3b).

Sannohe Y, Endo M, Katsuda Y, Hidaka K, Sugiyama H (2010) J Am Chem Soc 132:16311– 16313 44. Xu Y, Sato H, Sannohe Y, Shinohara K-I, Sugiyama H (2008) J Am Chem Soc 130:16470– 16471 45. Birkedal V, Dong M, Golas MM, Sander B, Andersen ES, Gothelf KV, Besenbacher F, Kjems J (2011) Microsc Res Techn 74:688–698 46. Steinhauer C, Jungmann R, Sobey TL, Simmel FC, Tinnefeld P (2009) Angew Chem Int Ed 48:8870–8873 47. Lin C, Jungmann R, Leifer AM, Li C, Levner D, Church GM, Shih WM, Yin P (2012) Nat Chem 4:832–839 48.

Download PDF sample

Rated 4.38 of 5 – based on 34 votes